70% industrial ethanol is the industry standard solvent because it possesses a unique polarity profile that maximizes yield while ensuring product safety. It acts as a comprehensive solvent, effectively dissolving both non-polar and polar bioactive compounds found in propolis while simultaneously functioning as an antimicrobial agent to prevent contamination during the extraction process.
The Core Takeaway: The selection of 70% ethanol represents an optimal chemical balance; it provides the necessary solvency to extract a full spectrum of active ingredients—ensuring the integrity of the extract's complex profile—while maintaining a sterile environment that inhibits microbial growth.
The Chemistry of Extraction Efficiency
Dual Solubility
Propolis is a complex substance containing a mixture of resins, waxes, and essential oils. 70% ethanol takes advantage of an excellent polarity range.
Because it is not purely alcohol nor purely water, this concentration can effectively dissolve non-polar compounds (like resins) and polar compounds simultaneously.
Ensuring Component Integrity
Using a solvent that targets only one type of compound would result in an incomplete extract.
By utilizing 70% ethanol, manufacturers ensure the integrity of the extract's components is preserved. This allows for the successful retrieval of key active ingredients, such as phenolic compounds and flavonoids, which are critical for the biological activity of the final product.
Process Safety and Standardization
Antimicrobial Properties
Beyond its role as a solvent, ethanol at this concentration serves a protective function.
The 70% concentration provides effective antimicrobial properties. This prevents microbial contamination from compromising the sample quality during the extraction process, acting as a built-in preservative measure during the reaction phase.
Controlled Industrial Environments
In standardized industrial extraction tanks, 70% ethanol is mixed with propolis under controlled temperature and agitation.
This standardized approach allows for the maximization of active substances while facilitating the filtration of excess beeswax and insoluble impurities. This ensures the final soluble matter content meets strict industrial standards.
Understanding the Trade-offs
While 70% ethanol is highly effective for general extraction, it is not the only variable to consider.
Toxicity and Safety Profiles
The choice of solvent directly impacts the toxicity of the final extract. Research indicates that alternatives like propylene glycol may cause vasodilation or tissue toxicity.
Conversely, while ethanol is effective, it may not be suitable for all demographics. Extraction using olive oil or water offers higher safety levels, though potentially different extraction yields.
The Importance of Standardization
Using non-standardized extraction methods can lead to drastic fluctuations in biological activity and toxicity levels (LD50 values).
Regardless of the solvent chosen, utilizing standardized equipment is critical to manage the significant variations in propolis composition caused by its geographical origin.
Making the Right Choice for Your Goal
When selecting a propolis extract or designing an extraction protocol, the intended end-user determines the best solvent method.
- If your primary focus is Maximum Potency: Prioritize 70% ethanol extraction to ensure the broadest range of polar and non-polar active ingredients are captured.
- If your primary focus is Sensitive Demographics (Infants/Pregnant Women): Prioritize water-based or natural vegetable oil extraction to ensure the product is gentle and harmless, avoiding alcohol entirely.
- If your primary focus is Safety and Consistency: Ensure the extract is produced using standardized industrial equipment to guarantee precise control over active ingredient yields and toxicity levels.
Ultimately, 70% ethanol is selected when the goal is a robust, broad-spectrum extract that balances chemical efficiency with process hygiene.
Summary Table:
| Feature | 70% Industrial Ethanol | Water / Natural Oils |
|---|---|---|
| Primary Role | Broad-spectrum solvent & Antimicrobial | Alcohol-free gentle solvent |
| Solubility | Dissolves both polar & non-polar compounds | Targets limited polar compounds |
| Best For | Maximum potency and industrial yield | Sensitive groups (infants/pregnancy) |
| Safety Focus | Built-in microbial protection | High physiological safety profile |
| End Result | High-integrity, standardized extract | Low-potency, safe consumable |
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References
- Eggi Pur Pinandita, Aurora Ivana Br Simanjuntak. POTENTIAL TRIGONA BEEKEEPING (Heterotrigona itama and Geniotrigona thoracica) AT SOUTH LABUHANBATU CITY, NORTH SUMATERA PROVINCE, INDONESIA. DOI: 10.21776/jtapro.2022.023.02.3
This article is also based on technical information from HonestBee Knowledge Base .
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