A sampling container equipped with a #8 mesh screen acts as a precise mechanical filter. It facilitates separation by exploiting the physical size difference between the host and the parasite. During the sampling process—typically an alcohol wash—the screen’s mesh openings are large enough to allow the dislodged Varroa mites and liquid to pass through into a collection chamber, yet small enough to retain the honeybees in the main compartment.
Core Takeaway The #8 mesh screen functions as a critical exclusionary barrier that automates the sorting process. By converting a manual inspection task into a mechanical filtration process, it ensures high sampling efficiency and accurate parasite quantification without the need to handle individual bees.
The Mechanics of Separation
The Principle of Size Exclusion
The effectiveness of the container relies on the geometry of the #8 mesh.
This specific wire spacing creates a physical barrier that is impassable for honeybees due to their body size. However, the openings are sufficiently wide to allow Varroa mites—which are significantly smaller—to pass through unimpeded.
The Role of Liquid and Agitation
In the standard alcohol wash method, the container is filled with a solution that dislodges the mites from the bees.
As the container is agitated, the liquid acts as a carrier medium. It washes the detached mites downward through the mesh screen and into the lower reservoir, while the bees remain tumbling above the screen.
Efficiency and Time Savings
Without a mesh screen, separating mites from a sample of 300 bees would require tedious manual sorting.
The integrated screen allows this separation to happen largely instantaneously during the washing process. This significantly reduces the labor required for field sampling and minimizes human error in counting.
Operational Variations
The Double-Layer Sieve Concept
Some advanced sampling systems utilize a double-layer approach based on filtration principles.
In these setups, an upper sieve (the #8 mesh) retains the bees, while a lower, finer sieve may be used to capture the mites while letting liquid drain away. This multi-stage filtration provides a clean baseline for quantitative analysis.
Non-Destructive Separation (Sugar Shake)
While the primary mechanism often involves alcohol, the same mesh physics apply to the "sugar shake" method.
In this scenario, powdered sugar coats the bees to dislodge mites. The mesh allows the sugar and mites to fall through when shaken, preserving the life of the bees for subsequent behavioral studies or return to the hive.
Understanding the Trade-offs
Sample Mortality vs. Preservation
The most efficient use of this hardware (the alcohol wash) is lethal to the bee sample.
While the mesh facilitates separation in both methods, utilizing alcohol ensures a higher mite drop rate (higher accuracy) but sacrifices the bees. The sugar shake method preserves the bees but is generally less consistent in dislodging every mite.
Debris Interference
The #8 mesh is not selective regarding what passes through, only what size passes through.
Pollen, small debris, or hive trash that is smaller than the mesh openings will also fall into the collection chamber. This can occasionally require the user to distinguish between mites and debris during the final count.
Making the Right Choice for Your Goal
To maximize the utility of a mesh-equipped sampling container, align your method with your specific data requirements:
- If your primary focus is Maximum Accuracy: Use the container with an alcohol solution to ensure the highest rate of mite detachment and the most reliable count, accepting the loss of the bee sample.
- If your primary focus is Colony Preservation: Use the container with the powdered sugar method to utilize the mesh for live separation, allowing you to return the bees to the hive after monitoring.
The #8 mesh screen transforms a complex biological sorting problem into a simple mechanical process, providing the consistency required for effective hive management.
Summary Table:
| Feature | #8 Mesh Screen Functionality |
|---|---|
| Mechanism | Size exclusion / Mechanical filtration |
| Bees | Retained above the screen (too large for mesh) |
| Mites | Pass through the mesh (small enough to fall) |
| Liquid Carrier | Alcohol or soapy water used to dislodge parasites |
| Efficiency | Automates separation of ~300 bees in seconds |
| Application | Alcohol wash (lethal) or Sugar shake (live) |
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References
- Álvaro Domínguez-Rebolledo, Henry Loeza-Concha. Identificación morfométrica de la especie predomínate de Varroa (Parasitiformes: Varroidae) en colonias de abejas en Hopelchén, Campeche. DOI: 10.21929/abavet2022.21
This article is also based on technical information from HonestBee Knowledge Base .
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