What Is The Procedure For Requeening A Bee Colony? Master The 24-Hour Rule For Hive Success

To requeen a bee colony, you must follow a strict timeline of removal and replacement. The procedure begins by locating, killing, and discarding the existing queen. You must then leave the colony entirely queenless for a waiting period of 24 hours before introducing the new queen inside her protective cage.

Successful requeening relies on the "queenless window"—a critical 24-hour period that allows the old queen's presence to fade. Skipping this step often leads to the colony rejecting or attacking the new monarch.

The Requeening Procedure

This process is designed to transition the colony's loyalty from the old matriarch to the new one with minimal disruption.

Locating the Old Queen

The first step is often the most difficult: you must find the current queen among thousands of workers. Once identified, she must be killed and discarded. Ensure she is removed completely from the apiary so her pheromones do not linger near the hive.

The 24-Hour Wait

Do not introduce the new queen immediately. The colony must be left queenless for exactly 24 hours. This waiting period allows the pheromones of the old queen to dissipate, signaling to the workers that they are truly queenless and in need of a replacement.

Introducing the Replacement

After the 24-hour period has passed, you may introduce the new queen. She should be placed into the hive while still inside her cage. This protects her from initial aggression while the workers acclimate to her specific scent.

Why Annual Requeening Matters

While the procedure requires care, the benefits of introducing a young queen are substantial for the long-term viability of the hive.

Increased Egg Production

Young queens are biologically more capable than their predecessors. They are more prolific egg-layers, which ensures a higher population of worker bees to sustain the colony.

Enhanced Pheromone Levels

Younger queens secrete significantly higher levels of pheromones. These chemical signals are the glue that holds the colony's social structure together.

Disease and Swarm Suppression

The robust pheromones of a new queen do more than just signal presence. They actively stimulate worker bees to forage, suppress the natural urge to swarm, and help suppress disease outbreaks within the hive.

Understanding the Risks

Timing is the most critical variable in this operation.

The Danger of Rushing

If the new queen is introduced before the 24-hour window closes, the workers may still sense the old queen's pheromones. This often results in the colony identifying the new queen as an intruder and killing her.

The Danger of Waiting Too Long

Conversely, leaving the colony queenless for significantly longer than 24 hours can cause panic. The workers may attempt to raise their own emergency queen from existing larvae, complicating the acceptance of the queen you purchased.

Making the Right Choice for Your Goals

Requeening is a management tool that should be aligned with your specific objectives for the apiary.

If your primary focus is maximum honey production: Prioritize annual requeening to ensure high egg-laying rates and stimulated foraging behavior.
If your primary focus is colony stability: Requeen to maintain high pheromone levels, which significantly reduces the risk of swarming and disease.

By adhering to the 24-hour rule and understanding the biological advantages of young queens, you ensure a smooth transition to a stronger, more resilient colony.

Summary Table:

Requeening Phase	Action Required	Duration / Timing	Purpose
1. Removal	Locate and remove the old queen	Day 0	Stop current pheromone production
2. Transition	Leave the colony queenless	24 Hours	Allow old pheromones to dissipate
3. Introduction	Insert new queen in cage	Day 1	Acclimatize workers to new scent
4. Release	Workers eat through candy plug	2-3 Days	Controlled release and acceptance

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