Knowledge Why are wide-mouth sampling jars used in Varroa mite extraction equipped with a wire mesh lid? Save Your Bees Now!
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Tech Team · HonestBee

Updated 2 days ago

Why are wide-mouth sampling jars used in Varroa mite extraction equipped with a wire mesh lid? Save Your Bees Now!


The wire mesh lid functions as a precision mechanical filter designed for selective separation. In the context of Varroa mite field extraction, specifically the powdered sugar roll method, this lid allows dislodged parasitic mites and excess sugar to pass through while retaining the larger host bees safely inside the jar.

Core Takeaway By utilizing a mesh aperture sized specifically between the dimensions of a Varroa mite and a honey bee, this device enables the non-destructive diagnosis of infestation levels. It allows beekeepers to quantify parasite load and physically separate the mites for counting without sacrificing the sample of bees.

The Mechanics of Physical Separation

The Role of Aperture Sizing

The effectiveness of the sampling jar relies entirely on the precision sizing of the wire mesh screen. The aperture (the gap between the wires) is engineered to create a specific physical barrier.

It must be large enough to allow Varroa mites—and the powdered sugar used to dislodge them—to fall through freely. Simultaneously, it must be small enough to act as an impassable containment wall for the adult honey bees.

Facilitating the Sugar Roll Method

In the powdered sugar roll method, sugar acts as a non-lethal friction agent. When the jar is shaken, the sugar dislodges the mites from the bees' bodies.

The mesh lid transforms the jar into a sieve during this vigorous shaking process. As the mites detach, gravity and centrifugal force drive them through the mesh and into a collection tray, leaving the cleaned bees behind.

The Non-Destructive Advantage

Preserving the Colony Workforce

The primary reason for using a mesh lid rather than a solid or fully open container is to ensure the safety of the host sample. This design adheres to the principles of non-destructive monitoring.

Because the bees are physically retained by the mesh while the parasites are removed, the colony does not lose approximately 300 bees (the standard sample size) per test.

Returning Live Bees

Once the shaking process is complete and the infestation rate has been calculated from the mites in the tray, the bees remaining in the jar can be returned to the colony.

While they may be coated in residual sugar, they remain alive and functional, allowing beekeepers to monitor resistance and infestation levels frequently without harming the overall health of the hive.

Understanding the Trade-offs

The Necessity of Vigorous Agitation

While the mesh lid protects the bees from escaping, the method requires vigorous shaking to force the mites through the screen.

If the shaking is too gentle, mites may remain trapped inside the jar with the bees, leading to a false low count. Conversely, excessively violent shaking can stress or injure the bees despite the protection of the mesh.

Mesh Integrity and Sizing

The reliability of the test is strictly bound to the integrity of the mesh. If the wire grid is damaged or if the aperture size is incorrect (e.g., too small for mites to pass or too large, allowing bees to squeeze through), the diagnostic results will be invalid.

Making the Right Choice for Your Goal

To ensure accurate Varroa monitoring, ensure your equipment aligns with your specific management objectives:

  • If your primary focus is Colony Preservation: Verify that your sampling jar utilizes a mesh lid that explicitly supports the "Sugar Roll" or "Sugar Shake" method to ensure bees can be returned to the hive alive.
  • If your primary focus is Diagnostic Accuracy: Ensure the mesh is free of debris and clumping sugar before every test, as blockages will prevent mites from passing through to the collection tray.

The wire mesh lid is the critical component that transforms a simple jar into a specialized tool for balancing accurate parasite monitoring with bee conservation.

Summary Table:

Feature Function & Purpose
Aperture Size Large enough for mites/sugar; small enough to retain honey bees.
Selective Filtration Acts as a mechanical sieve to separate dislodged mites from hosts.
Non-Destructive Allows bees to be returned to the colony alive after testing.
Sieve Efficiency Facilitates vigorous shaking without losing the sample bees.

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References

  1. Richard A. Giliba, Mathew Mpanda. Changing climate patterns risk the spread of Varroa destructor infestation of African honey bees in Tanzania. DOI: 10.1186/s13717-020-00247-4

This article is also based on technical information from HonestBee Knowledge Base .

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